Population studies have recently revealed that some of the major clonal lineages of Toxoplasma gondii dominate in different geographical areas. The lineage of Type II and III are widespread in all continents and dominate in Europe, Africa and North America. In addition, type 12 lineage is the most common type of wildlife in North America, Africa 1 and 3 is one of the main species in Africa, and ToxoDB PCR-RFLP # 9 is the kind of big in China. Overall strains of T. gondii were more diverse in South America than any other region. Here, we analyzed 164 isolates of T. gondii from three Central American countries (Guatemala, Nicaragua, Costa Rica), from one country in the Caribbean (Grenada) and five countries of South America (Venezuela, Colombia, Peru, Chile, and Argentina ).
The multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyping based on 11 polymorphic markers (SAG1, SAG2, alt.SAG2, SAG3, BtuB, GRA6, L358, PK1, C22-8, C29-2 and Apico) applied for 148 domestic poultry (Gallus domesticus) isolates and 16 isolates from the domestic cat (Felis catus) in Colombia; 42 genotypes were identified. Linkage disequilibrium analysis showed genetic recombination more frequently in populations of Nicaragua and Colombia, and to a lesser degree in the population of Costa Rica and Argentina. Bayesian structural analysis identified at least three groups of genetic and phylogenetic network analysis identifies four main groups. The ToxoDB PCR-RFLP # 7, Type III and II major lineages identified from Central and South America, with a high frequency of ToxoDB PCR-RFLP # 7 and Type III is closely related lineages.
direct molecular blood test for early Lyme disease can not be sensitive because of the low number of outstanding Borrelia burgdorferi DNA. To address these challenges, we have developed a strategy and genotype sensitive to detect B. burgdorferi directly from whole blood collected during the initial patient visit. This strategy increases sensitivity by using 1.25 mL of whole blood, a novel pre-enrichment of the entire specimen extracts to Borrelia multi-locus DNA prior to PCR and electrospray ionization mass spectrometry detection assay. We evaluate the blood test were collected at initial presentation of 21 patients of endemic areas who have both physician-diagnosed erythema migrans (EM) and serology two positive level either at baseline or follow-up visit after three weeks of antibiotic therapy.
Molecular genotyping of Toxoplasma gondii from Central and South America revealed high diversity within and between populations.
molecular genotyping of methicillin-resistant Staphylococcus aureus through enhanced fluorofor repetitive-sequence-PCR.
methicillin resistance in Staphylococcus aureus is a frequent cause of nosocomial infections and community acquired. Accurate, fast epidemiological typing is very important to identify the source and spread of infectious diseases and can provide detailed information about the generation of methicillin-resistant S. aureus (MRSA) strains. A high level of genetic linkage strains of MRSA have barred the use of the more conventional methods of genetic fingerprinting. A DNA fingerprinting method that exploit the rapid PCR amplification of repetitive DNA sequences in MRSA described.
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system.
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system.
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system.
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system.
Description: Granulocyte Macrophage Colony stimulating Factor (GM-CSF) is a cytokine that stimulates the colony formation of granulocyte-macrophage progenitors. GM-CSF is a pleiotropic cytokine and a member of endogenous cytokine family of the hematopoietic system.
Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.
Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues.
Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Canine GM-CSF Recombinant Protein is purified GM-CSF produced in yeast.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Chicken GM-CSF Recombinant Protein is purified GM-CSF produced in yeast.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Equine GM-CSF Recombinant Protein is purified GM-CSF produced in yeast
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Human GM-CSF Recombinant Protein is purified GM-CSF produced in yeast.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Mouse GM-CSF Recombinant Protein is purified GM-CSF produced in yeast.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes and monocytes and is glycosylated in its mature form.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Swine GM-CSF Recombinant Protein is purified GM-CSF produced in yeast.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GM-CSF . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GM-CSF . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GM-CSF . This antibody is tested and proven to work in the following applications:
Immunogen information: Synthesized peptide derived from part region of human GM-CSF protein at amino acid sequence of 100-144
Applications tips:
Description: A polyclonal antibody for detection of GM-CSF from Human. This GM-CSF antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GM-CSF protein at amino acid sequence of 100-144
Immunogen information: Synthesized peptide derived from part region of human GM-CSF protein at amino acid sequence of 100-144
Applications tips:
Description: A polyclonal antibody for detection of GM-CSF from Human. This GM-CSF antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GM-CSF protein at amino acid sequence of 100-144
Immunogen information: Synthesized peptide derived from part region of human GM-CSF protein at amino acid sequence of 100-144
Applications tips:
Description: A polyclonal antibody for detection of GM-CSF from Human. This GM-CSF antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GM-CSF protein at amino acid sequence of 100-144
Distribution of random chromosome repeat sequence provides an ideal target for detecting DNA fragment patterns specific to individual MRSA strains. Two PCR fingerprinting method uses oligonucleotide primer based on a repeated sequence found in Mycoplasma pneumoniae is presented. Repetitive element sequence-based PCR (rep-PCR) and fluorofor-enhanced rep-PCR (FERP) can identify the strain of the MRSA epidemic in the background. Oligonucleotide primer combinations are labeled with different fluorescent dyes simultaneously allowed FERP fingerprints and detection of the mecA gene.
Kent
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