Posted in Antibodies , Assay Kits , Biology Cells , cDNA , Clia Kits , Culture Cells , Devices , DNA Templates , DNA Testing , Elisa Kits , Enzymes , Equipments , Exosomes , Gels , Isotypes , Medium & Serums , NATtrol Panel , Uncategorized
Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics.
The recent introduction of molecular methods has gained increasing acceptance as a powerful tool for epidemiology and phylogeny of tuberculosis (TB). In this study, the efficiency of using the molecular typing of mycobacterial interspersed repetitive unit (MIRUS) rated on a set of 116 clinical isolates of Mycobacterium tuberculosis complex of 11 different geographical origin. The result was compared with spoligotyping and variable number tandem repeat of DNA (VNTRs) data type. Eighty-nine Miru different profiles obtained in the studied sample. Spoligotyping- or VNTR cluster-defined, divided into subclusters by Miru typing.
In contrast, nearly all clinical isolates clustered by MIRUS shown belong to cluster-based spoligotyping defined. Calculation of discriminatory power by Hunter-Gaston index (HGI) for VNTR, spoligotyping and Miru typing gives values, respectively, 0.959, 0.965 and 0.988, indicating high discriminatory power of MIRUS. Allelic diversity samples are measured for each Miru-VNTR loci; five Miru locus (Miru nos. 10, 23, 26, 31 and 40) are “very discriminant”, four (Miru nos. 4, 16, 24 and 39) are “fairly discriminant”, and three (Miru nos. 2, 20 and 27) is “bad discriminant”. Among the three VNTRs complementary (exact tandem repeats ETR-A, ETR and ETR-B-C), ETR-A is the most discriminant locus. A combined numerical analysis of spoligotyping, VNTR typing and the results corroborated Miru most recent evolutionary scenario for M. tuberculosis complex hypothesis.
M. canettii will be the first branch that has deviated from the general M. tuberculosis complex ancestors. East-Africa India (EAI) clade could be the first family to have diverged thereafter. A third branching separating M. africanum-M. bovis clade, followed by a separate node versus non-Beijing Beijing M. tuberculosis. Beijing is a different clade of Central Asia 1 (CAS1) family. Among the non-Beijing strains, branches such as Latin America and the Mediterranean (LAM), X and Haarlem clades diverged later. In conclusion, the results obtained showed concordance between clades defined by spoligotyping, and Miru-VNTR, and underscores the potential of this method for M. tuberculosis phylogeny reconstruction. We also conclude that the Miru typing is a very promising method that can be used in genotyping strategy “PCR-based two”, in conjunction with conventional epidemiological investigation.
Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics.
molecular epidemiological analysis of Cryptosporidium spp. in the UK: results of the genotype of Cryptosporidium spp. in 1705 from human stool samples and 105 samples of droppings from livestock.
Cryptosporidium present in stool samples of 1,705 people and 105 livestock were analyzed by PCR-restriction fragment length polymorphism Cryptosporidium oocyst wall protein. Overall, genotype 1 (exclusive type of man) was detected in 37.8% of samples from humans, and genotype 2 (broad host range) was detected in 61.5%, the third designated genotype genotype 3 (Cryptosporidium meleagridis) detected at 0, 3%, and both genotypes 1 and 2 have been recovered from 0.4%. All samples from cattle produce genotype 2.
Rabbit anti Bovine
E61I02501
EnoGene
0.1mg
EUR 225
Goat anti Bovine IgG
41C-CB0136
Fitzgerald
5 mg
EUR 229
Description: Goat anti Bovine IgG secondary antibody
Sheep anti Bovine IgA
41R-SB001
Fitzgerald
1 mg
EUR 172
Description: Sheep anti Bovine IgA secondary antibody
Sheep anti Bovine IgM
40C-CB0143
Fitzgerald
1 mg
EUR 191
Description: Sheep anti Bovine IgM secondary antibody
Sheep anti Bovine IgG1
20R-IS001
Fitzgerald
1 mg
EUR 218
Description: Sheep anti Bovine IgG1 antibody
Sheep anti Bovine IgG1
41R-IS001
Fitzgerald
1 mg
EUR 218
Description: Affinity purified Sheep polyclonal Sheep anti Bovine IgG1 antibody
Rabbit anti Bovine IgA
41R-RB001
Fitzgerald
1 mg
EUR 172
Description: Rabbit anti Bovine IgA secondary antibody
Rabbit anti Bovine IgG
40C-CB0115
Fitzgerald
2 mg
EUR 265
Description: Rabbit anti Bovine IgG secondary antibody
Rabbit anti Bovine IgG
40C-CB0129
Fitzgerald
5 mg
EUR 333
Description: Rabbit anti Bovine IgG secondary antibody
Goat anti Bovine IgG (HRP)
43C-CB0141
Fitzgerald
2 mg
EUR 335
Description: Goat anti Bovine IgG secondary antibody (HRP)
Goat anti Bovine IgG (FITC)
43C-CB0123
Fitzgerald
2 mg
EUR 322
Description: Goat anti Bovine IgG secondary antibody (FITC)
Goat anti Bovine IgG (FITC)
43C-CB0137
Fitzgerald
2 mg
EUR 257
Description: Goat anti Bovine IgG secondary antibody (FITC)
Sheep anti Bovine IgM (HRP)
43C-CB0145
Fitzgerald
1 mg
EUR 266
Description: Sheep anti Bovine IgM secondary antibody (HRP)
Sheep anti Bovine IgM (HRP)
43R-IS004hrp
Fitzgerald
1 mg
EUR 276
Description: Sheep anti Bovine IgM secondary antibody (HRP)
Anti- Transferrin Bovine Antibody
GWB-EB5591
GenWay Biotech
1 ml
Ask for price
Rabbit anti Bovine IgG (HRP)
43C-CB0120
Fitzgerald
1.5 mg
EUR 369
Description: Rabbit anti Bovine IgG secondary antibody (HRP)
Rabbit anti Bovine IgG (HRP)
43C-CB0134
Fitzgerald
2 mg
EUR 358
Description: Rabbit anti Bovine IgG secondary antibody (HRP)
Sheep anti Bovine IgG1 (HRP)
43R-IS002hrp
Fitzgerald
1 mg
EUR 277
Description: Sheep anti Bovine IgG1 secondary antibody (HRP)
Sheep anti Bovine IgG1 (HRP)
43R-IS003hrp
Fitzgerald
1 mg
EUR 253
Description: Sheep anti Bovine IgG1 secondary antibody (HRP)
Rabbit anti Bovine IgG (FITC)
43C-CB0116
Fitzgerald
1.5 mg
EUR 320
Description: Rabbit anti Bovine IgG secondary antibody (FITC)
Rabbit anti Bovine IgG (FITC)
43C-CB0130
Fitzgerald
2 mg
EUR 275
Description: Rabbit anti Bovine IgG secondary antibody (FITC)
Goat anti Bovine IgG (biotin)
43C-CB0140
Fitzgerald
2 mg
EUR 321
Description: Goat anti Bovine IgG secondary antibody (biotin)
Rabbit anti Bovine IgG (biotin)
43C-CB0119
Fitzgerald
1.5 mg
EUR 351
Description: Rabbit anti Bovine IgG secondary antibody (biotin)
Rabbit anti Bovine IgG (biotin)
43C-CB0133
Fitzgerald
2 mg
EUR 320
Description: Rabbit anti Bovine IgG secondary antibody (biotin)
Bovine Anti-RV ELISA Kit
EBA0830
Abclonal
96Tests
EUR 625.2
Bovine Anti-TG ELISA Kit
EBA0901
Abclonal
96Tests
EUR 625.2
Bovine Anti-β2GP1 ELISA Kit
EBG0284
Abclonal
96Tests
EUR 625.2
Goat anti Bovine IgG (rhodamine)
43C-CB0138
Fitzgerald
2 mg
EUR 257
Description: Goat anti Bovine IgG secondary antibody (Rhodamine)
Bovine Anti-GLS ELISA Kit
EBG0344
Abclonal
96Tests
EUR 625.2
Bovine anti-PF4 ELISA Kit
EBH0254
Abclonal
96Tests
EUR 625.2
Bovine Anti-Jo1 ELISA Kit
EBJ0009
Abclonal
96Tests
EUR 625.2
Bovine anti-LC1 ELISA Kit
EBL0217
Abclonal
96Tests
EUR 625.2
Bovine anti-PR3 ELISA Kit
EBP0718
Abclonal
96Tests
EUR 625.2
Bovine anti-TPO ELISA Kit
EBT0064
Abclonal
96Tests
EUR 625.2
Bovine Anti-MCV ELISA Kit
EBA0036
Abclonal
96Tests
EUR 625.2
Bovine Anti-CFG ELISA Kit
EBA0037
Abclonal
96Tests
EUR 625.2
Bovine Anti-C1q ELISA Kit
EBA0424
Abclonal
96Tests
EUR 625.2
Bovine Anti-GAD ELISA Kit
EBA0427
Abclonal
96Tests
EUR 625.2
Bovine Anti-RNP ELISA Kit
EBA0429
Abclonal
96Tests
EUR 625.2
Bovine Anti-GM1 ELISA Kit
EBA0444
Abclonal
96Tests
EUR 625.2
Bovine Anti-MOG ELISA Kit
EBA0478
Abclonal
96Tests
EUR 625.2
Bovine Anti-HAV ELISA Kit
EBA0822
Abclonal
96Tests
EUR 625.2
Bovine Anti-BIP ELISA Kit
EBB0037
Abclonal
96Tests
EUR 625.2
Bovine Anti-Bpi ELISA Kit
EBB0692
Abclonal
96Tests
EUR 625.2
Bovine anti-CTS ELISA Kit
EBC0702
Abclonal
96Tests
EUR 625.2
Bovine Anti-DNA ELISA Kit
EBD0216
Abclonal
96Tests
EUR 625.2
Rabbit anti Bovine Serum antibody
40R-RB001
Fitzgerald
2 ml
EUR 273
Description: Rabbit anti Bovine Serum antibody
Goat anti Bovine IgG (H + L)
41C-CB0108
Fitzgerald
2 mg
EUR 115
Description: Goat anti Bovine IgG (H + L) secondary antibody
Goat anti Bovine IgG (H + L)
41R-IG004
Fitzgerald
1.5 mg
EUR 223
Description: Goat anti Bovine IgG (H + L) secondary antibody
Bovine anti-Sp17 ELISA Kit
EBS0281
Abclonal
96Tests
EUR 625.2
Bovine Anti-TPOR ELISA Kit
EBT0033
Abclonal
96Tests
EUR 625.2
Bovine Anti-AT1R ELISA Kit
EBA0116
Abclonal
96Tests
EUR 625.2
Bovine Anti-Surv ELISA Kit
EBA0425
Abclonal
96Tests
EUR 625.2
Bovine Anti-RRNP ELISA Kit
EBA0431
Abclonal
96Tests
EUR 625.2
Bovine Anti-AChR ELISA Kit
EBA0453
Abclonal
96Tests
EUR 625.2
Bovine anti-EBMZ ELISA Kit
EBE0128
Abclonal
96Tests
EUR 625.2
Bovine anti-vgpc ELISA Kit
NSL1419Bo
Sunlong
96 T
EUR 528
Bovine Anti-TA Anti-body ELISA Kit
NSL1951Bo
Sunlong
96 T
EUR 528
Rabbit anti Bovine IgG (rhodamine)
43C-CB0117
Fitzgerald
1.5 mg
EUR 275
Description: Rabbit anti Bovine IgG secondary antibody (Rhodamine)
Rabbit anti Bovine IgG (rhodamine)
43C-CB0131
Fitzgerald
2 mg
EUR 275
Description: Rabbit anti Bovine IgG secondary antibody (Rhodamine)
Bovine Anti trypsin ELISA kit
E01A78925
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti-livin ELISA Kit
EBA0030
Abclonal
96Tests
EUR 625.2
Bovine Anti-AT2R1 ELISA Kit
EBA0118
Abclonal
96Tests
EUR 625.2
Bovine Anti-CIIAb ELISA Kit
EBA0421
Abclonal
96Tests
EUR 625.2
Bovine Anti-ssDNA ELISA Kit
EBA0426
Abclonal
96Tests
EUR 625.2
Bovine Anti-DsDNA ELISA Kit
EBA0447
Abclonal
96Tests
EUR 625.2
Bovine Anti-RVIgG ELISA Kit
EBA0831
Abclonal
96Tests
EUR 625.2
Bovine Anti-RVIgM ELISA Kit
EBA0832
Abclonal
96Tests
EUR 625.2
Bovine anti-dsDNA ELISA Kit
EBD0011
Abclonal
96Tests
EUR 625.2
Bovine Anti-bovine serum albumin IgM ELISA kit
E01A78671
BlueGene
96T
EUR 700
Description: ELISA
Rabbit anti Bovine IgG (H + L)
40C-CB0101
Fitzgerald
2 mg
EUR 229
Description: Rabbit anti Bovine IgG (H + L) secondary antibody
Bovine anti-PANDER ELISA Kit
EBP0032
Abclonal
96Tests
EUR 625.2
Bovine Anti-toxIgG ELISA Kit
EBA0833
Abclonal
96Tests
EUR 625.2
Bovine Anti-toxIgM ELISA Kit
EBA0834
Abclonal
96Tests
EUR 625.2
Goat anti Bovine IgG (Alk Phos)
43C-CB0142
Fitzgerald
1 mg
EUR 362
Description: Goat anti Bovine IgG secondary antibody (Alk Phos)
Anti- Catalase (Bovine Liver) Antibody
GWB-8A2071
GenWay Biotech
50 mg
Ask for price
Anti- Arginase (Bovine Liver) Antibody
GWB-738907
GenWay Biotech
50 mg
Ask for price
Bovine Anti-typhus Anti-body ELISA Kit
NSL1965Bo
Sunlong
96 T
EUR 528
Goat anti Bovine IgG (Texas Red)
43C-CB0139
Fitzgerald
2 mg
EUR 302
Description: Goat anti Bovine IgG secondary antibody
Anti- Transferrin Bovine -HRP Antibody
GWB-4DEE13
GenWay Biotech
1 ml
Ask for price
Rabbit anti Bovine IgG (Alk Phos)
43C-CB0121
Fitzgerald
1 mg
EUR 376
Description: Rabbit anti Bovine IgG secondary antibody (Alk Phos)
Rabbit anti Bovine IgG (Alk Phos)
43C-CB0135
Fitzgerald
1 mg
EUR 369
Description: Rabbit anti Bovine IgG secondary antibody (Alk Phos)
Bovine Anti Prothrombin ELISA kit
E01A78586
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti-Aromatase ELISA kit
E01A80099
BlueGene
96T
EUR 700
Description: ELISA
Anti-CATALASE (Bovine Liver) Antibody
GWB-5B36D2
GenWay Biotech
2 mL
Ask for price
Bovine Anti-EJ-Anti-body ELISA Kit
NSL1903Bo
Sunlong
96 T
EUR 528
Bovine Anti-OJ-Anti-body ELISA Kit
NSL1923Bo
Sunlong
96 T
EUR 528
Bovine Anti-Monocyte Anti-body ELISA Kit
NSL1789Bo
Sunlong
96 T
EUR 528
Rabbit anti Bovine IgG (Texas Red)
43C-CB0118
Fitzgerald
1.5 mg
EUR 351
Description: Rabbit anti Bovine IgG secondary antibody
Rabbit anti Bovine IgG (Texas Red)
43C-CB0132
Fitzgerald
2 mg
EUR 355
Description: Rabbit anti Bovine IgG secondary antibody
Bovine Anti-Mi2-Anti-body ELISA Kit
NSL1771Bo
Sunlong
96 T
EUR 528
Bovine Anti-PL7-Anti-body ELISA Kit
NSL1931Bo
Sunlong
96 T
EUR 528
Bovine Anti-nucleolus Anti-body ELISA Kit
NSL1921Bo
Sunlong
96 T
EUR 528
Bovine Anti-PL12-antibody ELISA
E01A85599
BlueGene
96T
EUR 700
Description: ELISA
Anti- Beta Lactoglobulin Bovine Antibody
GWB-77CA4C
GenWay Biotech
1 mg
Ask for price
Bovine Anti-PL12-Anti-body ELISA Kit
NSL1930Bo
Sunlong
96 T
EUR 528
Bovine Anti-macrophage Anti-body ELISA Kit
NSL1808Bo
Sunlong
96 T
EUR 528
Bovine anti Ku antibody ELISA kit
E01A79989
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti EJ antibody ELISA kit
E01A82806
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti hnRNP K ELISA kit
E01A78711
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti-RV IgA ELISA Kit
EBV0014
Abclonal
96Tests
EUR 625.2
Bovine Anti-RV IgG ELISA Kit
EBV0015
Abclonal
96Tests
EUR 625.2
Bovine Anti-RV IgD ELISA Kit
EBV0016
Abclonal
96Tests
EUR 625.2
Bovine Anti-RV IgM ELISA Kit
EBV0021
Abclonal
96Tests
EUR 625.2
Bovine Anti-Q-Ab ELISA Kit
EBA0827
Abclonal
96Tests
EUR 625.2
Bovine Anti-Q-Ab ELISA Kit
NSL1936Bo
Sunlong
96 T
EUR 528
Bovine Anti-sp100-Anti-body ELISA Kit
NSL1945Bo
Sunlong
96 T
EUR 528
Bovine Anti P62 Antibody ELISA kit
E01A78676
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti G2s antibody ELISA kit
E01A78703
BlueGene
96T
EUR 700
Description: ELISA
Bovine anti PL7 antibody ELISA kit
E01A79065
BlueGene
96T
EUR 700
Description: ELISA
Bovine anti IgA antibody ELISA kit
E01A79983
BlueGene
96T
EUR 700
Description: ELISA
Bovine anti Mi2 antibody ELISA kit
E01A79991
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti-AMW IgM ELISA Kit
EBA0821
Abclonal
96Tests
EUR 625.2
Goat anti Bovine IgG (H + L) (HRP)
43C-CB0113
Fitzgerald
2 ml
EUR 270
Description: Goat anti Bovine IgG (H + L) secondary antibody (HRP)
Goat anti Bovine IgG (H + L) (HRP)
43C-CCB0113
Fitzgerald
2 mL
EUR 349.2
Description: Goat anti Bovine IgG (H + L) secondary antibody (HRP)
Goat anti Bovine IgG (H + L) (HRP)
43R-IG035hrp
Fitzgerald
1.5 ml
EUR 519
Description: Goat anti Bovine IgG (H + L) secondary antibody (HRP)
Goat anti Bovine IgG (H + L) (HRP)
43R-IG039hrp
Fitzgerald
1 mg
EUR 115
Description: Goat anti Bovine IgG (H + L) secondary antibody (HRP)
Anti- Alkaline Phosphatase Bovine Antibody
GWB-174B57
GenWay Biotech
1 ml
Ask for price
Bovine Anti kein antibody ELISA kit
E01A79988
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti-keIn Anti-body(AKA) ELISA Kit
NSL1770Bo
Sunlong
96 T
EUR 528
Bovine Anti-red cell Anti-body ELISA Kit
NSL1937Bo
Sunlong
96 T
EUR 528
Bovine Anti-Skin Anti-body(ASA) ELISA Kit
NSL1943Bo
Sunlong
96 T
EUR 528
Bovine Anti-PM-Scl ELISA Kit
EBP0698
Abclonal
96Tests
EUR 625.2
Bovine Anti-SCL70 ab ELISA Kit
NSL1941Bo
Sunlong
96 T
EUR 528
Goat anti Bovine IgG (H + L) (FITC)
43C-CB0109
Fitzgerald
2 mg
EUR 152
Description: Goat anti Bovine IgG (H + L) secondary antibody (FITC)
Goat anti Bovine IgG (H + L) (FITC)
43R-IG033ft
Fitzgerald
2 mg
EUR 206
Description: Goat anti Bovine IgG (H + L) secondary antibody (FITC)
Bovine Anti-carbamylated-FCS ELISA
E01A80053
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti livin antibody ELISA kit
E01A78574
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti Actin Antibody ELISA kit
E01A78686
BlueGene
96T
EUR 700
Description: ELISA
Bovine anti sp100 antibody ELISA kit
E01A80019
BlueGene
96T
EUR 700
Description: ELISA
Bovine Anti titin antibody ELISA kit
E01A86781
BlueGene
96T
EUR 700
Description: ELISA
Among the 469 patients who were infected during the eight drinking water-related outbreaks, five outbreaks mainly occurred due to genotype 1, and three are due to genotype 2. Fifty-four samples collected from patients involved with five -associated plague swimming pool: two outbreaks are due to genotype 1, one is due to genotype 2, and the remaining two involved both genotype 1 and 2. among the 26 plague outbreak nurseries family an d 1 children (2 to 3 members per group) , each genotype were recovered from different members of each outbreak: 13 is due to genotype 1, and 14 are due to genotype 2.